《人肌紅蛋白基因外顯子一的生物信息學(xué)分析》由會(huì)員分享，可在線閱讀，更多相關(guān)《人肌紅蛋白基因外顯子一的生物信息學(xué)分析（18頁(yè)珍藏版）》請(qǐng)?jiān)谘b配圖網(wǎng)上搜索。

1、人肌紅蛋白?基因外顯子?一的生物信?息學(xué)分析 一． Intro?ducti?on：Myogl?obin, the artic?le is compo?sed of a pepti?de chain?s and a bindi?ng prote?in of heme prost?hetic? group?, is withi?n the muscl?e oxyge?n stora?ge prote?in, its oxyge?n satur?ation? of the ellip?tic curve?.Myogl?obin is the main prote?in of skele?tal m

2、uscl?e and cardi?ac muscl?e, when muscl?e damag?e, leaka?ge to the circu?latin?g in the blood? in muscl?e tissu?e, incre?ase the serum? conce?ntrat?ion of myogl?obin, the index? is used to deter?mine wheth?er muscl?e damag?e. 二． Purpo?se：對(duì)人肌紅蛋?白基因外顯?子一基因序?列使用 “Bioed?it和DN?AMAN、anthe?5.0”及相關(guān)網(wǎng)站?進(jìn)行GC含

3、?量統(tǒng)計(jì)、電子酶切、引物設(shè)計(jì)、基因在人類?基因組中的?定位、電子克隆及?蛋白在細(xì)胞?內(nèi)的定位、氨基酸組成?統(tǒng)計(jì)、分子量、等電點(diǎn)、親疏水性、信號(hào)肽分析?（跨膜區(qū)域）、二級(jí)結(jié)構(gòu)（含保守結(jié)構(gòu)?域）及三維結(jié)構(gòu)?分析，miR分析?，啟動(dòng)子分析?等。其中抗原表?位分析、多序列比對(duì)?和進(jìn)化分析?等。 三． The natur?e and funct?ion of myogl?obin： Natur?e: myogl?obin = a polyp?eptid?e chain? + a prost?hetic? group? polyp?eptid?e chain?, compo?sed of 153 a

4、mino? acid resid?ues Prost?hetic? group?: heme prost?hetic? group? Molec?ular weigh?t: 16, 700 Funct?ion: trans?port of oxyge?n and oxyge?n stora?ge funct?ion in muscl?e terti?ary struc?ture of myogl?obin 四． Key word: 肌紅蛋白（myogl?obin） 五． Imple?ments? and metho?ds： 采用Bio?edit和?DNAMA?N分析軟件?工具和在線

5、?有關(guān)蛋白質(zhì)?和核酸方面?的分析軟件?，分別進(jìn)行核?酸和蛋白質(zhì)?兩個(gè)方面的?分析。 核酸分析首?先從pub?med核酸?數(shù)據(jù)庫(kù)中搜?索到人的M?yoglo?bin核酸?序列，分別進(jìn)行序?列的基本性?質(zhì)分析，限制性酶譜?分析及其基?因在染色體?上的定位分?析。具體操作方?法將根據(jù)上?述各個(gè)軟件?上“Help”文件進(jìn)行，基因在染色?體上的定位?分析根據(jù)網(wǎng)?頁(yè)上的操作?說(shuō)明進(jìn)行。 從pubm?ed蛋白質(zhì)?數(shù)據(jù)庫(kù)中搜?索到人的M?yoglo?bin和整?個(gè)前體My?oglob?in蛋白質(zhì)?序列，分別進(jìn)行蛋?白序列的基?本性質(zhì)分析?（氨基酸組成?、分子量、等電點(diǎn)），親疏水性分?析，蛋白酶切位?點(diǎn)分析

6、，信號(hào)肽序列?分析，二級(jí)結(jié)構(gòu)和?三維結(jié)構(gòu)分?析，同源序列和?進(jìn)化分析。二級(jí)結(jié)構(gòu)分?析包括結(jié)構(gòu)?功能域、Motif?、保守位點(diǎn)等?，具體操作方?法將根據(jù)上?述各個(gè)軟件?上“Help”文件和網(wǎng)頁(yè)?上的操作說(shuō)?明進(jìn)行。 Reslu?ts: 1.從NCBI?中可以得到?一下有關(guān)M?yoglo?bi蛋白分?子的信息： Human?myogl?obingene, exon 1 GenBa?nk: M1009?0.1 LOCUS? HUMMG?I1 2552 bp DNA linea?r PRI 07-JAN-1995 DEFIN

7、?ITION? Human? myogl?obin gene, exon 1. ACCES?SION M1009?0 VERSI?ON M1009?0.1 GI:18758?0 KEYWO?RDS Fok famil?y repet?itive? seque?nce; direc?t repea?t; myogl?obin; repea?t regio?n; tande?m repea?t. SEGME?NT 1 of 3 SOURC?E Homo sapie?ns (human?) ORGAN?ISM Hom

8、o sapie?ns Eukar?yota; Metaz?oa; Chord?ata; Crani?ata; Verte?brata?; Eutel?eosto?mi; Mamma?lia; Euthe?ria; Euarc?honto?glire?s; Prima?tes; Haplo?rrhin?i; Catar?rhini?; Homin?idae; Homo. REFER?ENCE 1 (bases? 1 to 2552) AUTHO?RS Akabo?shi,E. TITLE?

9、 Cloni?ng of the human? myogl?obin gene JOURN?AL Gene 33 (3), 241-249 (1985) PUBME?D 29890?88 COMME?NT Origi?nal sourc?e text: Human?: fetal? liver? DNA (libra?ry of Lawn et al.), clone? lambd?a-HMB15?1; delta?-thala?ssemi?a leuko?cyte DNA (libra?ry of Kimu

10、r?a et al.), clone? lambd?a-HMB15?9. Draft? entry? and compu?ter-reada?ble copy of seque?nce [1] kindl?y provi?ded by E.Akabo?shi, 22-OCT-1985. A tande?m repea?t seque?nce is found? at posit?ion 142-794. Posit?ion of the mRNA cap site was deduc?ed

11、 from that of the seal myogl?obin gene (Blanc?hetot? et al. 1983). FEATU?RES Locat?ion/Quali?fiers? sourc?e 1..2552 /organ?ism="Homo sapie?ns" /mol_t?ype="genom?ic DNA" /db_xr?ef="taxon?:9606"

12、 /map="22q11?.2-qter" repea?t_reg?ion 1100..1750 /note="Fok famil?y repet?itive? eleme?nt" prim_?trans?cript? 1895..>2552 /note="myogl?obin mRNA and intro?n" gene join(1965..2552,M1460?2.1:1..2671,M1460?3

13、.1:1..501) /gene="MB" CDS join(1965..2059,M1460?2.1:1415..1637,M1460?3.1:355..501) /gene="MB" /note="myogl?obin" /codon?_star?t=1 /prote?in_id?="AAA59?595.1"

14、 /db_xr?ef="GI:38687?2" /trans?latio?n="MGLSD?GEWQL?VLNVW?GKVEA?DIPGH?GQEVL?IRLFK?GHPET?LEKF DKFKH?LKSED?EMKAS?EDLKK?HGATV?LTALG?GILKK?KGHHE?AEIKP?LAQSH?ATKHK?IPV KYLEF?ISECI?IQVLQ?SKHPG?DFGAD?AEGAM?NKALE?LFRKD?MASNY?KELGF?QG"

15、 exon <1965..2059 /gene="MB" /note="myogl?obin; G00-119-378" /numbe?r=1 intro?n 2060..>2552 /gene="MB" /note="myogl?obin intro?n A" ORIGI?N 2 bp upstr?ea

16、m of RsaI site. 1 gtact?gtatt? ttcat?tcctc? ttagt?tatct? cccta?aaaag? actct?gagtt? ccttg?aacac? 61 aggaa?ggtgt? tttat?ttgat? tttgt?tatcc? tcagc?atgta? gcagt?gtctg? acaca?cagta? 121 ggtgc?tctat? cactg?tgaga? gggat?ggatg? gatgg?gtgga? gttac?agatg? gatag?aagga? 181 tagat?

17、ggagg? gatgg?gtgga? tgatg?gatgg? ataga?tggat? ggagg?gggga? tgatg?aatgg? 241 aggga?taatg? agtgg?atgaa? tgagg?gaatg? ggtgg?atgga? tggat?ggagg? gatgg?aggaa? 301 cagat?agata? gatgg?aggga? tgggt?gggtg? atgga?tggat? agatg?gatgg? aggga?gggat? 361 gatga?atgga? gggat?aatga? atgga?tgaat?

18、gaggg?gatgg? gtgga?tggat? gaatg?gaggg? 421 atgat?gggtg? gatga?atgaa? ttgag?ggatg? gatgg?atgaa? cacat?ggatg? gatgg?ataga? 481 tggat?agatg? gagga?actgg? tggat?tttgg? atgga?tgggt? ggatg?gatag? atgaa?tgaat? 541 gcctg?gatag? acaaa?gagat? gatgg?ataga? tgaat?agatg? aatta?aggga? tgtcg?g

19、atag? 601 atgga?gggat? tgata?gatgt? tggat?ggatg? ggtgg?tggat? ggata?gatga? gtgaa?tgcat? 661 ggata?gacaa? agaga?tgatg? gatgg?atgaa? ttaag?ggatg? acaga?tggat? ggatg?gatga? 721 gtaac?tggat? ggaca?agtgg? ataaa?tggat? agatg?gttga? atacc?tgaat? ggatt?gaagg? 781 aggat?gcatg? gat

20、gt?aagat? aaggc?taatc? atcct?ccact? ctctt?tcttt? gcaaa?accat? 841 ccacc?cattt? actca?ataaa? cattt?attca? gttca?aactt? ggcac?aaagc? accat?gtgag? 901 gccca?agaga? tacgt?gggtt? aataa?aacag? agctc?ctgcc? ctcct?gaaaa? ctgca?aagaa? 961 agggg?cgtgg? cttcc?tgagt? tcaaa?tccca? actct?gcca

21、g? cgact?agctg? tacat?cagtg? 1021 atgtt?tccct? acttt?ctctc? aatta?aatag? ggata?atgtc? agtac?ctatc? acatt?gggag? 1081 gtctt?gcggg? gatta?aatga? gttac?caaat? gccaa?gtgtt? tggga?caggg? cctgg?caccc? 1141 agcaa?agtct? cttgt?gagtg? ctggc?tgcta? ttatc?ctaat? ggaga?agatg? gcatg?aaaac?

22、 1201 cagga?aatag? gatgc?ccttt? gggaa?gcaat? gcaac?aggaa? cttac?acaaa? gaaag?gaaag? 1261 gagga?agcaa? ttagt?ggtgt? ctcaa?aggag? tatgt?caaga? aaaac?ttttc? agagg?gaaac? 1321 ctttg?agcag? ggtca?tgaaa? acagg?agttc? tctaa?gagat? tgtgg?acttg? cctgg?gacca? 1381 cctgg?ctata? agcac?aaaac?

23、catcc?ggttc? ctttc?tgtca? cttct?ggcgg? gtgag?gggtc? 1441 tctgg?caaag? gggca?gaagg? tgcgt?gagag? gttgc?gaatg? gccag?gactg? tcctg?gggcc? 1501 agccg?gggca? cctgg?tggcc? aagct?tagaa? acatg?acagg? tcctc?ttggg? agggc?tgacc? 1561 gcagg?gagcg? ttggg?tttca? ggctg?ctggc? gtcgg?cttct? gtggt?g

24、ccct? ttctg?tcggc? 1621 tatga?gagtc? cagac?agtgc? ccaac?ctcct? cccct?tcttt? ccaca?cgcac? aacca?cccca? 1681 ccccc?tgtgg? cctga?gctgt? cctgc?ctcgc? cacaa?tggca? cctgc?cctaa? aatag?cttcc? 1741 catgt?gaggg? ctaga?gaaag? gaaaa?gatta? gaccc?tccct? ggatg?agaga? gagaa?agtga? 1801 agg

25、ag?ggcag? gggag?gggga? cagcg?agcca? ttgag?cgatc? tttgt?caagc? atccc?agaag? 1861 gtata?aaaac? gccct?tggga? ccagg?cagcc? tcaaa?cccca? gctgt?tgggg? ccagg?acacc? 1921 cagtg?agccc? atact?tgctc? ttttt?gtctt? cttca?gactg? cgcca?tgggg? ctcag?cgacg? 1981 gggaa?tggca? gttgg?tgctg? aacgt?ctgg

26、g? ggaag?gtgga? ggctg?acatc? ccagg?ccatg? 2041 ggcag?gaagt? cctca?tcagg? taaaa?ggaag? agatt?ccatt? gcccc?tgcca? cccac?accct? 2101 aagat?caagg? gtgtt?cagct? gcaag?gtgga? aagtt?tgcac? gtggg?gtagg? tcagt?tggct? 2161 gcatt?agtta? agggt?gttag? aacgg?tcact? tgctt?tttct? ttgct?tttaa? gtgt

27、c?aggga? 2221 ttgga?ctcag? gagag?ggaaa? ggagc?cattt? caggc?tgatg? tcagc?agctg? gagga?agcat? 2281 gagaa?tcaaa? cctag?gatgc? tcaga?gtcca? ccagg?aagaa? tttta?gaatt? ataga?cagtc? 2341 agagt?taaca? agggt?cctga? gagat?tttgt? acagc?cacct? ctctt?acagg? atgag?gacaa? 2401 aaagc?gactg?

28、agaag?gggag? gacat?ttcca? gagtc?acagc? tcatt?aaatg? ctctt?aaagt? 2461 gtcaa?ggtta? agaca?tgctc? ttcaa?gggga? gacag?atctg? gttct?agact? tggct?ctgcc? 2521 actga?gccac? tgggt?gacct? ttggg?aaggt? ac Offic?ial Symbo?l MBpro?vided? byHGNC Offic?ial Full Name myogl?obinp?rovid?ed byHGNC Pr

29、ima?ry sourc?e :HGNC:6915 Locus? tag :RP4-569D1?9.7 Seere?lated?：Ensem?bl:ENSG0?00001?98125?;HPRD:01170?;MIM:16000?0;Vega:OTTHU?MG000?00150?606 Gene type:prote?in codin?g Refse?p statu?s:REVIE?WED Organ?ism：Homo sapie?ns Linea?ge： Eukar?yota; Metaz?oa; Chord?ata; Crani?ata; Verte?brata?; Eu

30、tel?eosto?mi; Mamma?lia; Euthe?ria; Euarc?honto?glire?s; Prima?tes; Haplo?rrhin?i; Catar?rhini?; Homin?idae; Homo Also known? as：PVALB? Genom?ic conte?xt Locat?ion: 22q13?.1 Seque?nce: Chrom?osome?: 22; NC_00?0022.10 (36002?811..36019?401, compl?ement?) See MB inEpige?nomic?s,MapVi?ewer Chro

31、m?osome? 22 - NC_00?0022.10 Genom?ic regio?ns, trans?cript?s, and produ?cts Genom?icSeq?uence? Go to nucle?otide?Graph?icsFASTA?GenBa?nk NC_00?0022.10:36M..36M(22Kbp?) C Find on Seque?nce:

32、 - + Tools? Confi?gure 2. 利用Pri?mer premi?er5.0軟件對(duì)m?yoglo?bin核酸?序列進(jìn)行引?物設(shè)計(jì)其結(jié)?果如下： Sense? prime?r: 5 TACTC?GTGCC?TTTCG?TACTA?GGCTC? 3 Anti-sense? prime?r: 5 ATGAG?CACGG?AAAGC?ATGAT?CCGAG? 3 3. 蛋白質(zhì)序列?分析 利用ExP?ASy軟件?包對(duì)該基因?進(jìn)行氨基酸?組成統(tǒng)計(jì)、分子量、等電點(diǎn)等分?析及磷酸化?分析