《【病毒外文文獻(xiàn)】2006 Mosaic structure of human coronavirus NL63, one thousand years of evolution (1)》由會(huì)員分享，可在線閱讀，更多相關(guān)《【病毒外文文獻(xiàn)】2006 Mosaic structure of human coronavirus NL63, one thousand years of evolution (1)（1頁(yè)珍藏版）》請(qǐng)?jiān)谘b配圖網(wǎng)上搜索。

16 Journal of Clinical Virology 2006 Vol 36 suppl 2 I Identification of respiratory viruses detected during three consecutive winter seasons E Pagani 1 C Petters 1 D Secolo 1 M Casini 2 L Pescollderungg 3 B Moser 4 E Percivalle 5 H R Huemer 6 C Larcher 1 1Laboratory of Microbiology and Virology 2Department of Haematology 3Department of Pediatrics 4 Department of Intensive Care Medicine Bolzano General Hospital Bolzano Italy 5Servizio di Virologia IRCCS Policlinico San Matteo Pavia Italy Department of Hygiene Microbiology and Social Medicine Innsbruck Medical University Innsbruck Austria In order to identify the different viral agents more frequently involved in mild and severe respiratory tract infections 614 symptomatic patients 351 attending the pediatric and 263 the hematology units of the General Hospital of Bolzano Italy were investigated dur ing three consecutive seasons from October 2003 through April 2006 Bronchoalveolar lavage bronchial aspirate nasopharyngeal secretion and nasopharyngeal lavage specimens were examined by direct fluorescent antibody assay and where possible shell vial culture technique for the presence of influenza virus A B FluA B Para influenza virus 1 2 3 PIV1 2 3 Adenoviruses Adeno Res piratory syncytial virus RSV and for the last season human Metapneumovirus hMPV During the entire study period we identified 185 different viral infections 30 147 patients were infected by RSV 79 followed by 13 FluA 7 9 hMPV 5 9 PIV3 5 3 Adeno 2 2 PIV2 1 1 FluB 0 5 and 1 PlVl 0 5 Presence of the virus was confirmed in 7 out of the 9 hMPV positive samples by RT PCR Sequencing of the amplified hMPV NP gene fragments indicated a different genotype compared to the iso lates obtained in the nearby North Tyrolean region earlier Larcher et al JHLT 2005 The majority of the viruses have been detected in the pediatric patients however the PIV1 2 3 seem to be more frequent in the hematological patients While RSV remains the prevalent virus in the pediatric subgroup for every examined season this was not repeatedly observed in hematological patients Moreover while for the 2004 2005 season no PlY have been identified we did not detect presence of FluA B last season IP Mosaic structure of human coronavirus NL63 one thousand years of evolution L van der Hoek 1 R Dijkman 1 L Deng 2 M E Jebbink 1 H A Ross 2 B Berkhout 1 K Pyrc 1 1Department of Human Retrovirology Academic Medical Center University of Amsterdam Meibergdreef 15 1105 AZ Amsterdam The Netherlands 2School of Biological Sciences and Bioinformatics Institute University of Auckland Private Bag 92019 Auckland New Zealand Background and Aims Before the SARS CoV outbreak only 2 human coronaviruses HCoV were known HCoV OC43 and HCoV 229E In 2004 a fourth human coronavirus HCoV NL63 was dis covered in The Netherlands Little is known about the heterogeneity of HCoV NL63 isolates and its evolutionary characteristics Methods We determined the complete genome sequence of two HCoV NL63 clinical isolates designated 057 and 496 Furthermore of 21 additional NL63 isolates various genome fragments were analyzed to confirm phylogenetic clustering Results and Conclusions The genomes of the clinical isolates of HCoV NL63 are 27538 and 27550 nucleotides long and share the same genome organization We compared the new isolates with the prototype strains Amsterdam 1 and NL and observed 99 sequence similarity between isolates with 497 heterogeneous posi tions Clustering of these variable sites suggests the presence of two hypervariable regions in the la and S genes The lb and N genes were most conserved Phylogenetic analysis indicates the presence of HCoV NL63 variants with distinct genetic markers Bootscan analysis revealed that the genomes have a mosaic structure with multiple recombination sites Employing three different algorithms we assessed the mutation rate for the S gene of group Ib coronaviruses to be 3times 10 4 mutations per site per year and the split of HCoV NL63 and its closest relative HCoV 229E was dated back to the eleventh century Abstracts 9th ESCV Annual Meeting lP Diagnosis of human metapneumovirus by immunofluorescence the Newcastle experience C Taylor 1 Y Taha 1 B Young 1 G Toms 2 F Fenwick 2 R McGuckin 2 1Health Protection Agency Laboratory Newcastle Upon Tyne 2University of Newcastle Upon Tyne Newcastle Upon Tyne UK Background and Aims Human metapneumovirus hMPV was discovered in 2001 hMPV has a worldwide distribution and has a spectrum of respiratory illness in children similar to Respiratory Syncytial Virus RSV The aim of the study was to investigate the prevalence of hMPV infection in hospitalized patients in north east England during April 2005 to April 2006 using immunofloures cence IF Methods A polyclonal antiserum to hMPV was prepared and used in an indirect IF assay The reagent was validated against an in house hMPV RT PCR F and N gene targets IF was also performed using a pool of monoclonal antibodies and compared with the polyclonal antiserum Results Indirect IF using the polyclonal antiserum had a sensi tivity of 89 percent and a sensitivity of 96 percent when validated against RT PCR 857 samples were tested by both polyclonal and monoclonal antibody reagents with a concordance of 99 8 percent 1860 respiratory samples were tested of which 52 percent were from children aged less than 1 year The mean overall positivity rate for hMPV was 5 percent hMPV was the second most prevalent virus after RSV with 92 and 343 positive samples respectively The peak months of hMPV prevalence were November to March Conclusions hMPV is widely prevalent in hospitalized patients in the north east of England and was the second most commonly diagnosed virus after RSV Diagnosis of hMPV by indirect IF was sensitive specific and practical The monoclonal antibody pool and polyclonal antiserum were equally effective in diagnosing hMPV by IE lP Detection of human metapneumovirus in clinical specimens using a novel immunoassay K Barry Murphy 1 T Sloots 2 S Setterquist 3 G Gray 3 S Reid 1 G Elliott 1 1Biotrin International Ltd 93 The Rise Mount Merrion Co Dublin Ireland 2Queensland Paediatric Infectious Diseases Laboratory Royal Children s Hospital Brisbane Queensland Australia 3Department of Epidemiology College of Public Health University of Iowa 200 Hawkins Dr C21K GH Iowa City IA 52242 USA Background and Aims Human Metapneumovirus hMPV has been described as a causative agent for acute respiratory tract in fections and is considered one of the main causes of hospitalisation for such infections in young children worldwide There is currently a requirement for an antigen detection assay for respiratory screening Here we describe an antigen detection EIA utilising a combination of monoclonal antibodies directed to major hMPV proteins Methods The performance characteristics of the immunoassay system were evaluated using panels of PCR confirmed hMPV positive or negative respiratory specimens from two separate clini cal laboratories Results The prototype assay results exhibited close correlation to PCR data A high level of specificity was demonstrated with a panel of potentially cross reactive non hMPV specimens The newly developed immunoassay has been found to be reactive with hMPV subgroups 1 and 2 of genotypes A and B from viral culture Conclusions This efficient highly sensitive and specific assay will have useful implications for hMPV detection in respiratory spec imens and or in culture confirmation